2024 Cell signaling tech - p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...

 
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411.. Cell signaling tech

Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Place the tubes on the magnetic rack until the solution turns clear (30 sec to 2 min) and then remove the liquid. Remove tubes from magnetic rack. Add 50 µl of pAG-MNase pre-mix to each tube and gently mix the sample by pipetting up and down. Incubate tubes at 4°C for 1 …Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under …In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).Our goal is to understand how signaling pathways are integrated at the molecular and systems levels to control cellular responses. We have two main focuses: First, we study …Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min. Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb. Western blot analysis of extracts from CAD cells and neonatal mouse brain using Synapsin-1 (D12G5) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from CAD cells using Synapsin-1 (D12G5) XP Rabbit mAb #5297. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Montminy, M. (1997) Annu Rev Biochem 66, 807-22. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, …Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP … Refine Results. In the CST video library, you can watch how-to videos from the Tech Tips series to improve your technique, research webinars presented by scientists from CST and other institutions, and videos relevant for specific applications or disease research areas. Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Monoclonal Antibody for studying EEA1. Cited in 345 publications. Validated for WB, IP, IF, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, EEA1 (C45B10) Rabbit Monoclonal Antibody (CST #3288) is ready to ship. Product Description. This Cell Signaling Technology antibody is tested in-house for indirect flow cytometric analysis in human cells and is expected to react with cell surface expressed CARs of varying specificity, which contain a G4S linker within the scFv of the extracellular domain. Cell Signaling Technology (CST) | 21,261 followers on LinkedIn. CST is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools. | Founded by ... Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected]. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9). Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Show More. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2).CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and … ELISA: An Introduction. Enzyme-linked immunosorbent assay (ELISA) allows for the quantification of a molecule of interest using antibodies and tags (such as the enzyme horseradish peroxidase [HRP]), either directly or indirectly coupled to the antibody for detection. ELISA can be used with a variety of sample types, such as cell lysate, tissue ... Dana Seehale joined Cell Signaling Technology in February 2017 as Director, Lab Informatics, responsible for multiple technologies supporting Product Development and Production, including PDP/PLM, Core LIMS, and Jira. Her responsibilities were later expanded to include the PIM/STEP system and the Oracle EBS/EPortal platforms. Dana is now ...Our goal is to understand how signaling pathways are integrated at the molecular and systems levels to control cellular responses. We have two main focuses: First, we study …Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 … At Cell Signaling Technology (CST), we have developed a panel of detection reagents that are designed to recognize a broad range of CARs: Anti-CAR linker antibodies. These first-to-market reagents can be incorporated into multiparametric flow panels for monitoring CAR expression, trafficking, and persistence in preclinical models. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic ...CUT&RUN was performed with HeLa cells and Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina ® (ChIP …CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and …XP ® monoclonal antibodies are high quality rabbit monoclonal antibodies exclusively available from Cell Signaling Technology (CST). XP monoclonal antibodies are generated using XMT ® technology, a proprietary rabbit monoclonal method developed at CST. This technology provides access to a broad range of antibodies unattainable with …1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.Cell Signaling Technology (CST) a private company founded by scientist, understands your need to make decision with confidence, providing the best-in-class products and services. No vendor is more stringent about antibody validation than CST, so when you partner with us you get results you can trust faster, to move your oncology project forward ...Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Montminy, M. (1997) Annu Rev Biochem 66, 807-22. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, …Since 2000, Cell Signaling Technology (CST) has offered a rigorously tested and well-cited polyclona... Charles Farnsworth, PhD Aug 9, 2023. A Quick Guide to Neuronal and Glial Cell Type Markers. The central nervous system (CNS) is composed of highly specialized cells, each of which play a key r...Rab -Rabbit. All -All Species Expected. Polyclonal Antibody for studying H2AX (Ser139) phosphate. Cited in 1048 publications. Validated for WB, WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H2A.X (Ser139) Antibody (CST #2577) is ready to ship. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. IHC-validated Antibodies for Cellular Senescence. Accelerate and increase confidence in your research results with IHC-validated p16 INK4A (BC42). An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for antibody specificity and sensitivity. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. …Use the Cell Signaling Technology Technical Documentation search tool to find Product Data Sheets, Certificates of Analysis (CoA), and Safety Data Sheets (SDS). Begin your search by entering the product number in the search field. Online publication of this documentation makes it easier for you to access it, and more environmentally friendly ...Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards …Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. cerevisiae Ce-C. …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Rab -Rabbit. All -All Species Expected. Polyclonal Antibody for studying H2AX (Ser139) phosphate. Cited in 1048 publications. Validated for WB, WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H2A.X (Ser139) Antibody (CST #2577) is ready to ship. Product Description. This Cell Signaling Technology antibody is tested in-house for indirect flow cytometric analysis in human cells and is expected to react with cell surface expressed CARs of varying specificity, which contain a G4S linker within the scFv of the extracellular domain. Rab -Rabbit. All -All Species Expected. Polyclonal Antibody for studying H2AX (Ser139) phosphate. Cited in 1048 publications. Validated for WB, WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H2A.X (Ser139) Antibody (CST #2577) is ready to ship.The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&Tag was performed with Hep G2 cells treated with Thapsigargin #12758 (300nM) for 4h and ATF-4 (D4B8) Rabbit mAb, using CUT&Tag Assay Kit #77552.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Cell Signaling Technology has an overall rating of 4.5 out of 5, based on over 132 reviews left anonymously by employees. 96% of employees would recommend working at Cell Signaling Technology to a friend and 96% have a positive outlook for the business. This rating has improved by 2% over the last 12 months.The innate immune system mainly consists of phagocytic cells, such as neutrophils, macrophages, natural killer cells, basophils, and others that target dangerous invading organisms. The cells of the adaptive immune system are T cells and B cells. Natural killer (NK) T cells have features of both innate and adaptive immune cells.Species Cross-Reactivity Key: Polyclonal Antibody for studying DRP1 (Ser616) phosphate. Cited in 343 publications. Validated for WB, IP, IF, F. Highly specific and rigorously validated in-house, Phospho-DRP1 (Ser616) Antibody (CST #3455) is ready to ship.The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.As we continue to create and provide the highest quality of products and services, be part of the team that empowers scientists and clinicians with our best-in-class tools and excellent customer experiences. Engage and share your expertise with our current customers while helping drive results by converting potential clients into profitable ones.Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. …Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Mar 14, 2023 · A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ... Poster: Signaling Pathways | Cell Signaling Technology. This poster provides a comprehensive overview of the major signaling pathways involved in cell growth, survival, differentiation, and stress responses. Download the PDF to explore the key molecules and interactions that regulate these pathways and their implications for human health and …Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411.Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb. Simple Western™ analysis of lysates (0.01 mg/mL) from HeLa cells using GAPDH (14C10) Rabbit mAb #2118. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. Videos & Webinars | Cell Signaling Technology. USA. Technical Support +1-877-678-8324. Contact Us. PhosphoSitePlus ®. Register Sign In. Quick Add.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Specificity / Sensitivity. Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.Place the tubes on the magnetic rack until the solution turns clear (30 sec to 2 min) and then remove the liquid. Remove tubes from magnetic rack. Add 50 µl of pAG-MNase pre-mix to each tube and gently mix the sample by pipetting up and down. Incubate tubes at 4°C for 1 …DIVERSITY, EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture.Pathways and Diagrams. Quickly identify your next research target using the interactive pathways, diagrams, guides and cellular landscapes in each of these collections. These resources provide insights into relevant cellular processes, structures and related diseases to help you identify related proteins and cellular mechanisms to investigate ...Immunoprecipitation of TIM-3 from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is TIM-3 (D5D5R ™) XP ® Rabbit mAb, #45208. Western blot was performed using TIM-3 (D5D5R ™) XP ® Rabbit mAb. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. Product Description. This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is ...The absence of signal in the HCT 116 knockout cells confirms the specificity of the antibody for LC3B. Western blot analysis of extracts from HeLa, C2C12, and KNRK cells, untreated (-) or treated with Chloroquine (50 μM, overnight; +) #14774 using LC3B (E5Q2K) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Lanes 1 & 2 are 5% input. Confocal immunofluorescent analysis of NCI-H1299 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-DRP1 (Ser616) Antibody (green). Actin filaments have been labeled with DY-554 phallodin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).The Harper Lab studies mechanisms underlying cellular homeostasis and signaling, with a focus on the ubiquitin system and the autophagy-lysosome system. The interest in the …Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3).Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells, transfected with a construct expressing HA-tagged Stat3 using HA-Tag (6E2) Mouse mAb #2367. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody.Van buren hotel, Washington mo movie theater, Tractor supply liberty tx, Camp cayuga, Cabela's billings montana, Otago university otago, Zahn dental, Andersons bookshop, Fortunecoinscasino, Dcs arizona, Epicair, Albrights, Sius, Harvest bakery

If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on …. Orthopedic clinic monroe la

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Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min. Background. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ ... Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and … If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality ...Specificity / Sensitivity. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases.Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Confocal immunofluorescent images of HUVEC cells untreated (left) or stimulated with Vascular Endothelial Growth Factor (VEGF) #9943 (right) and labeled with Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (top, green) and VEGF Receptor 2 (55B11) Rabbit mAb (bottom, green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is β3-Tubulin (D71G9) XP ® Rabbit mAb. Western blot analysis was performed using β3-Tubulin (D71G9) XP ® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody. Our goal is to understand how signaling pathways are integrated at the molecular and systems levels to control cellular responses. We have two main focuses: First, we study …Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.Polyclonal Antibody for studying H3 (Ser10) phosphate. Cited in 1110 publications. Validated for WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) Antibody (CST #9701) is ready to ship.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Our goal is to understand how signaling pathways are integrated at the molecular and systems levels to control cellular responses. We have two main focuses: First, we study …Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality ...Receptors are the main switches initiating downstream short-range and long-distance signaling in the cell and modulating cellular activities (Czyzewicz et al. … Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. XP ® monoclonal antibodies are high quality rabbit monoclonal antibodies exclusively available from Cell Signaling Technology (CST). XP monoclonal antibodies are generated using XMT ® technology, a proprietary rabbit monoclonal method developed at CST. This technology provides access to a broad range of antibodies unattainable with …Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for ... Support Information. Please select the team or topic you would like to reach out to for support: Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Cell Signaling Technology (CST) | 21,261 followers on LinkedIn. CST is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools. | Founded by ...At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.Dana Seehale joined Cell Signaling Technology in February 2017 as Director, Lab Informatics, responsible for multiple technologies supporting Product Development and Production, including PDP/PLM, Core LIMS, and Jira. Her responsibilities were later expanded to include the PIM/STEP system and the Oracle EBS/EPortal platforms. Dana is now ...Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates ...Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM ... GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis (2). Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons (3). In addition, some therapy-resistant cancer cells depend on GPX4 to survive. Immunohistochemistry (IHC) staining allows for 2 broad classes of detection: 1) chromogenic and 2) fluorescent. For chromogenic detection, CST recommends using polymer-based systems that avoid the limitations of the biotin-based system while also increasing sensitivity of the assay. Try SignalStain ® Boost IHC Detection Reagent (HRP, …Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.If you're looking for a career that you can feel good about with a company that cares about you, the environment, and our communities, look no further than Cell Signaling Technology. Check out our current career …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 and TCRβ . While you know that each protein is found at the plasma membrane, you need to check whether the antibodies recognize extracellular or intracellular portions of each. If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).Polyclonal Antibody for studying Flag-Tag. Cited in 774 publications. Validated for WB, IP, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #2368) is …Cell biology research seeks to understand all aspects of cell growth, survival, and death in the contexts of both health and disease. Understanding the complex and dynamic cellular landscape can provide insights into healthy and disease states. Cell Signaling Technology (CST) provides a diverse and comprehensive catalog of rigorously tested and ... Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein.Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ... GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis (2). Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons (3). In addition, some therapy-resistant cancer cells depend on GPX4 to survive. During medical school, he did research at the Rockefeller University. His postdoctoral fellowship was at the Whitehead Institute/Massachusetts Institute of Technology. The …The Harper Lab studies mechanisms underlying cellular homeostasis and signaling, with a focus on the ubiquitin system and the autophagy-lysosome system. The interest in the …Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).RSS Feed. Cell signalling is the mechanism by which stimuli are transmitted via a signalling cascade to effector molecules that orchestrate the appropriate response. Types of cell signalling ...The Harper Lab studies mechanisms underlying cellular homeostasis and signaling, with a focus on the ubiquitin system and the autophagy-lysosome system. The interest in the …Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).Cell Signaling Technology has an overall rating of 4.5 out of 5, based on over 132 reviews left anonymously by employees. 96% of employees would recommend working at Cell Signaling Technology to a friend and 96% have a positive outlook for the business. This rating has improved by 2% over the last 12 months.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and Phospho-CREB (Ser133) (87G3) Rabbit mAb, using CUT&RUN Assay Kit #86652.Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb #5174. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Immunoprecipitation of Bax from HepG2 cells. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Bax (D2E11) Rabbit mAb, #5023. Western blot was performed … Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Our goal is to understand how signaling pathways are integrated at the molecular and systems levels to control cellular responses. We have two main focuses: First, we study …Immunoprecipitation of NF-kB p65 from CHO cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is NF-κB p65 (D14E12) XP ® Rabbit mAb, #8242. Western blot was performed using NF-κB p65 (L8F6) Mouse mAb, #6956. GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis (2). Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons (3). In addition, some therapy-resistant cancer cells depend on GPX4 to survive. Immunoprecipitation of Bax from HepG2 cells. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Bax (D2E11) Rabbit mAb, #5023. Western blot was performed …Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 …Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP …NOTE: Most antibodies from Cell Signaling Technology work optimally between 1 and 2 ug per IP sample. In the case where there are multiple samples with varying concentrations, …Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. 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